Student Research at Duke

For my first week in Dr. Kuehn’s lab, I have focused primarily on technique.  So far I have learned how to extract and purify mRNA from both lung epithelial (LA-4) and RAW cells.  After this step, I converted the mRNA to cDNA, which I later purified in anticipation of running a “real-time” PCR.  Unfortunately, my findings are not currently accessible, however the figure above does represent how significant results may be depicted through this method.  On the other hand, knowing that I have produced consistent and expected results in the trial runs I have done this week has increased my excitement for the research I will begin on Monday.  I plan to investigate which genes become active when cells are exposed to Psudomonas vesicles, so that their detrimental health affects may be better understood, prevented, and eventually, cured.

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