Archive for July, 2007

And so it ends…

Wednesday, July 25th, 2007

These eight weeks have gone by far faster than I ever imagined was possible. And I’m happy to say that it was a truly awesome experience! I finished my poster late Sunday night, a little frustrated by the lack of conclusive data I had to present. If only I could stay another couple of weeks to finish out the second group of mice, I might be able to see something significant emerge from the data.

But reflecting on the summer, I’m starting to realize that it was “less about the results and more about the process” as a labmate of mine so wisely put it. (Well, maybe that’s not exactly true in the real world when you have to worry about publishing and getting grants, but it works for me.) I feel so lucky to have had this opportunity to be a part of the lab team. I’ve learned a lot, and I don’t just mean how to handle mice or the fastest way to clean out mouse poop from the activity boxes or even where the mice are most likely to hide when they’re trying to escape you (although I can assure you, I certainly learned those lessons quickly.) This summer I got my first real look at the scientific method in action, and though it’s not as simple as it seemed in seventh grade science class, it sure is a whole lot more interesting. It’s full of mistakes and unseen obstacles that you have to work around and problem-solve. And what was really cool was that I learned more than just the small task I was assigned to. I got to hear from my coworkers at lab meetings, I talked to fellow Howard Hughes Research Fellows to find out about their projects, and I sat through many talks from Duke faculty on a wide variety of topics within the large umbrella of biological research. At the beginning of each seminar, the lecturers were asked to tell their story about how they got to be where they are today, and I must say it was encouraging to learn that not everyone was born working in a laboratory. The program was phenomenal and geared exactly toward people like me - considering a career in science, but unsure how to go about it. I’m especially grateful to both the West and Williams labs for their help along the way and for patiently answering all my questions. I had a terrific time and I certainly hope this won’t be my last encounter with biological research.

And if you’re in the area and would like to see the awesome poster that I worked so hard on, come to the poster session this Friday, July 27th, 10:30-12 at the LSRC Hall of Science (next to Blue Express). It should be great - 30 HH Research Fellows plus about 20 more participants from different programs.

What I Want to Be When I Grow Up

Monday, July 16th, 2007

How many times have I been asked this question? It seems like the answer never gets any easier. From circus clown to veterinarian, my job aspirations have varied greatly over the years. Now that I’m in college, my plans have changed yet again. The more I learn about the field, the more I come to realize that biology is by far the most fascinating subject I’ve ever studied.

I’ve already eliminated the possibility of a career in medicine, which seems to be the plan of the vast majority of bio majors at Duke – I’m not terribly interested in the clinical applications and small-scale individual problems so much as the big picture. And after having the unbelievable opportunity this summer to participate in the Research Fellows program, I think biological research may be just the thing for me.

name of imageIn writing my application for the program, I had initially thought I might want to get involved in behavioral studies, hence my job working with the mice. However, while the line between biology and psychology is certainly interesting, after some exposure in the West lab, which deals strictly on a molecular scale, I think I may enjoy that equally as much, if not more. I am hoping in the course of my three years left at Duke to make the most of the time I have and to take advantage of the wide range of research opportunities in search of that one area of biology I will spend my life absorbed in. And if that doesn’t work out I suppose I can always fall back on one of my many previous dream jobs, though hopefully I won’t end up as a clown.

Research Update

Saturday, July 7th, 2007

Eight weeks certainly seemed like a long time when I first arrived in Durham, but now at the end of the fifth week, I’m starting to realize just how slow the research process is. We are finishing up our second week of activity boxes today and the results are encouraging. Initially the activity boxes were meant to be used as a means of determining whether the mice were capable of performing in the later mazes; name of imagehowever, after running the number through Excel and making LOTS of graphs, we think there may be a difference in the knockout mice’s activity levels during the day versus at night.

So the next step is going to be… more testing! We’ll be running the mice through what’s called an “elevated plus maze” in which two of the arms of the maze are blocked off by high walls whereas the other two arms are completely open. The idea of the maze is to test for anxiety by measuring how much time is spent in the dark, closed-off arms as opposed to the open arms (which to the mice are pretty scary). We’re hoping to see if maybe the differences in activity level that we noted are due to either an elevated or depressed level of anxiety. In other news, we’re lucky to have a new cohort of mice coming in this next week to add to our numbers. As of now, we have only been working with 23 mice, so it’s been difficult finding any significant differences. With only three weeks left, we’ve still got a lot to do - plus mazes and water mazes for the older mice, as well as running the younger rats through both the SHIRPA screens and activity boxes! Yikes!