Archive for July, 2007

My Career Plans

Wednesday, July 18th, 2007

            As the Howard Hughes Fellowship comes to an end, I am glad to say that I have more insight into what my future plans will be.  All throughout high school, I believed that I wanted to attend medical school and be a practicing physician.  However, there was always a lingering voice in my head that said that research was what I really wanted to do.  Having had zero research experience prior to Howard Hughes, I faced a great difficulty in deciding what was best for me.  Fortunately, I had the opportunity to do research this summer, and although I still am not one hundred percent sure of anything yet, I am definitely heading in the right direction.

            One thing I have learned this summer is that research can be very, very tedious at times.  Often a researcher spends more time performing redundant but necessary experiments than contemplating an actual research question.  One may notice from all of my posts that I talk about western blots quite a bit.  Well, the reason for that is not so much that I just love western blotting, it’s that I have to do so many!  This     aspect of life in research definitely forces me to pause and think if a career in research differs from what I actually want to do.  I do not know if I want to be pipetting for years to come! However, the one aspect of research that I truly believe diverges from my true interests is how narrow and specific research projects usually are.  For example, much research has been done in the area of determining molecular/signaling pathways for cells.  While such knowledge is certainly interesting and necessary to advancing overall scientific knowledge, I am not so sure that it is what I want to study.  I believe that I would be more interested in studying organisms on a larger scale, such as a specific organ system, versus what specific molecules or chemicals are involved in one specific process in the brain.

At this point I feel that I am more inclined to pursue a medical career as clinician, clinical researcher, or possibly both.  The reason for this is that I believe that results from this type of job are more tangible and easily appreciated, although no less valuable than the results of researchers.  That said, I still do not know for sure if what I feel know will still apply in two years.  These eight weeks of research I will complete, while a great experience, are hardly sufficient to truly grasp what research is all about.  Many beginning researchers such as myself are given simpler tasks to do at first before they can move on the more advanced techniques.  So, I hope to continue doing research, hopefully in the same lab, throughout my undergraduate years.  More experience in the lab will help me decide for sure what I want.  Who knows, maybe I will decide that both a medical and a biological research career (M.D./Ph.D are in my future, although the many years of schooling required may just be enough of a discouragement to prevent that from happening!  Hopefully in three years time all of my questions will be answered.

           

Status of the Rac

Friday, July 13th, 2007

Once again I must apologize for not being prompt with my blog posts. It seems like every day I say to myself, “I will blog today,” but then two weeks go by without anything being written. Anyway, last time I discussed how I intended to measure the activation of the protein Rac in Hela cells via cell stimulation with EGF. Well, the cells did finally grow to sufficient confluence and I made my cell lysate samples. I don’t think I have yet mentioned cell lysates, so basically they are samples made by destroying the cells but retaining all of the proteins and components in the cell. This experiment was run by doing what is called a Rac pulldown assay, in which the cell lysates are mixed with a substance called GST-PBD. GST-PBD selectively binds to activated Rac, so in this way we are able to isolate the active Rac and run the samples through a western blot (this I know I have mentioned!) to measure the activation.

Unfortunately, all the time spent making the lysate samples and running western blots proved to be fruitless, as the phosphor imaging scanner showed dirty protein membranes with absolutely no Rac bands. I have yet to determine what error caused this, as the scanner is in fact functioning correctly. This was a most frustrating occurrence, as it seemed that several days of work went down the drain.

After the unpleasantness, Hideji and I regrouped and decided to perform a simpler version of that experiment using 3T3 cells, which are fibroblast cells from mice. In some ways I am glad that I had to redo some of this experiment because I am finding that each time I make the lysates, along with doing the western blot, my technique gets a little better. This time the scans came out much better and I will be obtaining data next week by quantifying the Rac bands from the scans. Although I still have to “officially” analyze the scans, they look promising and I am very glad to end the week in this way.

Once again thank you for reading and although there are only two weeks left in the program I will give it my best effort to update my blog reguarly (well, maybe semi-regularly!).