Halfway Mark, Part 2
The week of Fourth of July is almost over, and luckily, the final preparations for one of my samples is almost done. I’ve been finishing up on a PCR modification (since our annealing temperatures were initially too high) and by next week we’ll hopefully get some results. As for the sample that did work, it’s been sitting in the incubator/shaker.
By next week, I’ll be able to retrieve the cells that overexpressed our modified ubiquitin. After that comes protein purification and SDS-PAGE gels.
Next week’s going to be busy.