The Second Month
This post starts off the second month of research under the HHMI program here at Duke University. In the previous month, I got to know my PI, my mentor, and the lab group, as well as various lab techniques. Cell cultures were started and we ordered primers for point-mutagenesis of ubiquitin. Now, in the second month of research, I’ll hopefully be transforming competent cells and overexpressing our modified ubiquitin to determine specifics about its structure and modes of function. But, as of now, certain PCRs don’t work and our sequencing data came back negative.
“Negative, Mr. Lian.”
But, Fourth of July week is upon us and the lab has quieted down somewhat, but for me, there is still work to be done.